First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association
Mem. Inst. Oswaldo Cruz
;
102(8): 919-924, Dec. 2007. ilus
Article
in English
| LILACS
| ID: lil-471856
ABSTRACT
We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
Full text:
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Index:
LILACS (Americas)
Main subject:
Pichia
/
Serratia
/
Bacillus
/
Aedes
/
Gastrointestinal Tract
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdades Integradas Maria Imaculada/BR
/
Universidade Estadual do Norte Fluminense Darcy Ribeiro/BR
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