Molecular characterization of astrovirus in stool samples from children in São Paulo, Brazil
Mem. Inst. Oswaldo Cruz
;
102(8): 969-974, Dec. 2007. graf, tab
Article
in English
| LILACS
| ID: lil-471864
ABSTRACT
The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2 percent) were positive 28/94 (29.7 percent) from children with acute diarrhea, 14/45 (31.1 percent) with persistent diarrhea, and 9/55 (16.3 percent) from control individuals. No data was available for 15/40 (37.5 percent) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6 percent) were positive 12/158 (7.6 percent) from individuals with acute diarrhea and 6/29 (20.7 percent) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2 percent) were characterized as human astrovirus type-1 (HAstV-1), two (3.0 percent) as HAstV-2, two (3.0 percent) as HAstV-3, and three (4.5 percent) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5 percent) was characterized as HAstV-1, six (33.3 percent) as HAstV-2, and one (5.5 percent) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mamastrovirus
/
Astroviridae Infections
/
Gastroenteritis
Type of study:
Diagnostic study
/
Observational study
/
Risk factors
Limits:
Child
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Becton Dickinson Indústrias Cirúrgicas Ltda/BR
/
Faculdade de Medicina do ABC/BR
/
Instituto Pasteur/BR
/
Universidade de São Paulo/BR
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