P53 gene mutation and protein expression in ameloblastomas
Braz. j. oral sci
;
5(17): 1034-1040, 2006. ilus, tab, graf
Article
in English
| LILACS, BBO
| ID: lil-472515
ABSTRACT
P53 gene is commonly found to be mutated in human cancer. Currently, limited data exist on the occurrence of p53 gene mutation in ameloblastomas. This study designed to evaluate the expression of p53 proteins and their gene mutations in human ameloblastomas. Four cases of epithelial odontogenic tumors were used. Normal cells of a 7 months aborted fetuss mandible were used as negative control. P53 Protein expression was detected immunohistochemically. DNA was extracted and amplified using PCR. Gene sequences analyzed to determine p53 mutation. p53 was overexpressed in one case of unicystic ameloblastoma and 2 cases of variant types of ameloblastoma. The Coding sequencing analyses demonstrated p53 gene alteration in cases of ameloblastoms in Exon 7 while, fetus epithelial mucosal cells showed the wild type DNA sequence in Exon 4. In conclusion p53 protein overexpression was detected by immunohistochemical staining as well as by DNA sequence analysis in cases of ameloblastomas.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunohistochemistry
/
Ameloblastoma
/
Mandible
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2006
Type:
Article
Affiliation country:
Malaysia
Institution/Affiliation country:
Universiti Sains Malaysia/MY
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