BOX-PCR-based identification of bacterial species belonging to Pseudomonas syringae: P. viridiflava group
Genet. mol. biol
;
31(1): 106-115, 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-476159
ABSTRACT
The phenotypic characteristics and genetic fingerprints of a collection of 120 bacterial strains, belonging to Pseudomonas syringae sensu lato group, P. viridiflava and reference bacteria were evaluated, with the aim of species identification. The numerical analysis of 119 nutritional characteristics did not show patterns that would help with identification. Regarding the genetic fingerprinting, the results of the present study supported the observation that BOX-PCR seems to be able to identify bacterial strains at species level. After numerical analyses of the bar-codes, all pathovars belonging to each one of the nine described genomospecies were clustered together at a distance of 0.72, and could be separated at genomic species level. Two P. syringae strains of unknown pathovars (CFBP 3650 and CFBP 3662) and the three P. syringae pv. actinidiae strains were grouped in two extra clusters and might eventually constitute two new species. This genomic species clustering was particularly evident for genomospecies 4, which gathered P. syringae pvs. atropurpurea, coronafaciens, garçae, oryzae, porri, striafaciens, and zizaniae at a noticeably low distance.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plant Diseases
/
Genome, Bacterial
/
Pseudomonas syringae
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2008
Type:
Article
Affiliation country:
Brazil
/
France
Institution/Affiliation country:
Embrapa Recursos Genéticos e Biotecnologia/BR
/
Institut National de la Recherche Agronomique/FR
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