Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
Braz. arch. biol. technol
;
50(6): 913-920, Nov. 2007. graf, tab
Article
in English
| LILACS
| ID: lil-476217
ABSTRACT
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 percent, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2007
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Dokuz Eylul University/TR
/
Ege University/TR
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