Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times
Mem. Inst. Oswaldo Cruz
;
103(1): 106-108, Feb. 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-478884
ABSTRACT
Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paleopathology
/
Ascariasis
/
Ascaris
/
DNA, Helminth
/
Cytochromes b
/
Feces
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
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