rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro
J. venom. anim. toxins incl. trop. dis
;
14(1): 113-127, 2008. graf, tab
Article
in English
| LILACS
| ID: lil-479343
ABSTRACT
Snake venom metalloproteases (SVMPs) comprise a family of snake venom toxins responsible for most of local and systemic effects observed during envenomation by snakes from the Viperidae family. The vascular system and more specifically the endothelium seem to be the preferential targets of these proteins. This work describes the effects of rACLF, a recombinant SVMP from Agkistrodon contortrix laticinctus on human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that rACLF activates HUVECs by the release of mediators involved in inflammation and hemostasis such as prostacyclin and interleukin-8. We also demonstrated that rACLF increased the expression of ICAM-I and decay accelerating factor (DAF). Moreover, rACLF protects the HUVECs against apoptosis induced by serum deprivation. These results suggest that the endothelial cell activation induced by SVMPs may have a significant role in the development of the local inflammatory lesion observed in Viperidae envenomation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Recombinant Proteins
/
Crotalid Venoms
/
Endothelial Cells
/
Metalloproteases
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Butantan Institute/BR
/
Federal University of São Carlos/BR
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