Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
Mem. Inst. Oswaldo Cruz
;
103(2): 191-194, Mar. 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-480633
ABSTRACT
Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ticks
/
DNA, Bacterial
/
Rickettsia felis
/
Siphonaptera
/
Insect Vectors
/
Animals, Domestic
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Secretaria Estadual de Saúde/BR
/
Universidade Federal de Ouro Preto/BR
/
Universidade Federal de Viçosa/BR
/
University of Texas Medical Branch/US
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