Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Genet. mol. res. (Online)
;
5(4): 851-855, 2006. ilus
Article
in English
| LILACS
| ID: lil-482073
ABSTRACT
DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yield from the hyphae of Crinipellis perniciosa, which causes witches' broom disease in cacao, from three other filamentous fungi, Lentinus edodes, Agaricus blazei, Trichoderma stromaticum, and from the yeast S. cerevisiae. Genomic DNA was suitable for PCR of specific actin primers of C. perniciosa, allowing it to be differentiated from fungal contaminants, including its natural competitor, T. stromaticum.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Fungal
/
Mycological Typing Techniques
/
Genome, Fungal
/
Agaricales
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Santa Cruz/BR
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