Histopathological characterization of a syngeneic orthotopic murine bladder cancer model
Int. braz. j. urol
;
34(2): 220-229, Mar.-Apr. 2008. ilus
Article
in English
| LILACS
| ID: lil-484455
ABSTRACT
PURPOSE: We developed and characterized by histopathology and immunohistochemistry a syngeneic murine bladder tumor model derived from the MB49 tumor cell line. MATERIALS AND METHODS: Bladder tumor implantation was achieved by intravesical instillation of 5 x 10(5) MB49 tumor cells in C57BL/6 mice. A chemical lesion of the bladder was performed in order to promote intravesical tumor implantation. The bladder wall lesion was accomplished by transurethral instillation of silver nitrate (AgNO3). After 15 days, the animals were sacrificed, examined macroscopically for intravesical tumor and bladder weight. Histology and immunohistochemistry were performed using cytokeratin 7 (CK7), carcinoembrionic antigen (Dako-CEA), p53 and c-erbB2 oncoprotein (Her2/neu). RESULTS: Twenty-nine out of 30 animals (96.7 percent) developed intravesical tumors in a 15-day period. Macroscopically, the mean bladder weight was 0.196g (0.069-0.538g), 10 to 15 times the normal bladder weight. The immunohistochemical analysis showed significant membrane expression of CEA and CK7: a similar finding for human urothelial cancer. We also characterized absence of expression of p53 and anti-Her2/neu in the murine model. CONCLUSIONS: High tumor take rates were achieved by using the chemical induction of the bladder tumor. Although electric cauterization is widely described in the literature for syngeneic orthotopic animal models, the technique described in this study represents an alternative for intravesical bladder tumor implantation. Moreover, the histopathology and immunohistochemical analysis of the murine bladder tumor model derived from the MB49 cell line showed a resemblance to human infiltrating urothelial carcinoma, allowing clinical inference from experimental immunotherapy testing.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Urinary Bladder Neoplasms
/
Carcinoma, Transitional Cell
/
Disease Models, Animal
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Ibirapuera University/BR
/
University of Sao Paulo/BR
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