Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification
J. venom. anim. toxins incl. trop. dis
;
14(2): 303-312, 2008. graf
Article
in English
| LILACS
| ID: lil-484566
ABSTRACT
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 14,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biotin
/
Avidin
/
Culicidae
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Adolfo Lutz Institute/BR
/
Pasteur Institute/BR
/
University of São Paulo/BR
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