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Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes): isolated isoforms and kinetics properties
Aquino-Silva, Maria Regina de; Schwantes, Maria Luiza Barcellos; Munin, Flavia Simone; Schwantes, Arno Rudi; Santos, Silvana Pereira dos.
  • Aquino-Silva, Maria Regina de; Universidade do Vale do Paraíba. Instituto de Pesquisa & Desenvolvimento. São José dos Campos. BR
  • Schwantes, Maria Luiza Barcellos; Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos. BR
  • Munin, Flavia Simone; Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos. BR
  • Schwantes, Arno Rudi; Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos. BR
  • Santos, Silvana Pereira dos; Universidade do Vale do Paraíba. Instituto de Pesquisa & Desenvolvimento. São José dos Campos. BR
Genet. mol. biol ; 31(1,suppl): 337-342, 2008. ilus, graf
Article in English | LILACS | ID: lil-484608
ABSTRACT
Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which would be caused by genetic drift.
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Full text: Available Index: LILACS (Americas) Main subject: Perciformes / Malate Dehydrogenase Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Carlos/BR / Universidade do Vale do Paraíba/BR

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Full text: Available Index: LILACS (Americas) Main subject: Perciformes / Malate Dehydrogenase Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Carlos/BR / Universidade do Vale do Paraíba/BR