Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae)
Genet. mol. biol
;
31(2): 532-538, 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-484993
ABSTRACT
We investigated inheritance and carried out gene fine mapping of aroma in crosses between the aromatic elite hybrid rice Oryza sativa indica variety Chuanxiang-29B (Ch-29B) and the non-aromatic rice O. sativa indica variety R2 and O. sativa japonica Lemont (Le). The F1 grains and leaves were non-aromatic while the F2 non-aroma to aroma segregation pattern was 31. The F3 segregation ratio was consistent with the expected 121 for a single recessive aroma gene in Ch-29B. Linkage analysis between simple sequence repeat (SSR) markers and the aroma locus for the aromatic F2 plants mapped the Ch-29B aroma gene to a chromosome 8 region flanked by SSR markers RM23120 at 0.52 cM and RM3459 at 1.23 cM, a replicate F2 population confirming these results. Three bacterial artificial chromosome (BAC) clones cover chromosome 8 markers RM23120 and RM3459. Our molecular mapping data from the two populations indicated that the aroma locus occurs in a 142.85 kb interval on BAC clones AP005301 or AP005537, implying that it might be the same gene reported by Bradbury et al (2005a; Plant Biotec J. 3363-370). The flanking markers Aro7, RM23120 and RM3459 identified by us could greatly accelerate the efficiency and precision of aromatic rice breeding programs.
Full text:
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Index:
LILACS (Americas)
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2008
Type:
Article
/
Project document
Affiliation country:
China
/
United States
Institution/Affiliation country:
Clemson University/US
/
Sichuan Academy of Agricultural Sciences/CN
/
Sichuan Agricultural University/CN
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