Genetic characterization of morphologically variant strains of Paracoccidioides brasiliensis
Mem. Inst. Oswaldo Cruz
;
103(3): 306-309, May 2008. ilus, graf
Article
in English
| LILACS
| ID: lil-485213
ABSTRACT
Molecular characterization of Paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based on the generated RAPD patterns placed the 14 P. brasiliensis strains into five groups with similarity coefficients of 72 percent. A high correlation between the genotypic and phenotypic characteristics of the strains was observed. A 750 bp-RAPD fragment found only in the wild-type phenotype strains was cloned and sequenced. Genetic similarity analysis using BLASTx suggested that this RAPD marker represents a putative domain of a hypothetical flavin-binding monooxygenase (FMO)-like protein of Neurospora crassa.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paracoccidioides
/
Genetic Variation
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
Affiliation country:
Brazil
/
United kingdom
Institution/Affiliation country:
Fiocruz/BR
/
Universidade de São Paulo/BR
/
University of St. Andrew/GB
/
University of St. Andrews/GB
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