Molecular characterization of enterococci harboring genotype and phenotype incongruence related to glycopeptide resistance isolated in Brazilian hospitals
Mem. Inst. Oswaldo Cruz
;
103(3): 301-305, May 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-485225
ABSTRACT
Three Enterococcus faecalis and one Enterococcus faecium strains were characterized by plasmid profile, pulsed-field gel electrophoresis (PFGE) and determination of antimicrobial minimal inhibitory concentrations. VanA elements were characterized by Long PCR, overlapping PCR and DNA sequencing. Enterococcal strains showed resistance to vancomycin and harbored the vanA gene, and three these were teicoplanin susceptible while one showed intermediate resistance to teicoplanin. Two E. faecalis strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis strains showed a deletion in the right terminal region of the Tn1546-like element. The E. faecium strain showed an insertion element in the vanXY intergenic region. Mutations in VanA elements were not found. Rearrangements in the VanA element could be responsible for incongruities in genotype and phenotype in these strains.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Vancomycin
/
Enterococcus faecium
/
Enterococcus faecalis
/
Teicoplanin
/
Anti-Bacterial Agents
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Adolfo Lutz/BR
/
Universidade de São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS