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Experimental carcinogenesis on the oropharyngeal mucosa of rats with hydrochloric acid, sodium nitrate and pepsin / Carcinogênese experimental na mucosa orofaríngea de ratos com ácido clorídrico, nitrato de sódio e pepsina
Del Negro, André; Araújo, Marina Raquel; Tincani, Alfio José; Meirelles, Luciana; Martins, Antônio Santos; Andreollo, Nelson Adami.
  • Del Negro, André; UNICAMP. Department of Surgery. Division of Head and Neck. BR
  • Araújo, Marina Raquel; UNICAMP. Laboratory of Enzymology and Experimental Carcinogenesis. BR
  • Tincani, Alfio José; UNICAMP. Department of Surgery. Division of Head and Neck. BR
  • Meirelles, Luciana; UNICAMP. Department of Pathology. BR
  • Martins, Antônio Santos; UNICAMP. Department of Surgery. Division of Head and Neck. BR
  • Andreollo, Nelson Adami; UNICAMP. Department of Surgery. Gastroenterological Surgery. BR
Acta cir. bras ; 23(4): 337-342, July-Aug. 2008. graf, tab
Article in English | LILACS | ID: lil-486170
ABSTRACT

PURPOSE:

To investigate the carcinogenic action of hydrochloric acid, pepsin and sodium nitrate on the oropharyngeal mucosa of rats, simulating the reflux of gastric contents.

METHODS:

Eighty-two Wistar rats were divided in seven groups and submitted to 2 or 3 weekly applications of hydrochloric acid, pepsin and sodium nitrate on the pharyngeal mucosa during six months. Study groups comprised 12 animals each. Rats in groups I and II were submitted to 2 (GI) or 3 (GII) weekly applications of 0.1N hydrochloric acid. Groups III and IV were submitted to 2 (GIII) or 3 (GIV) weekly applications of 0.1N hydrochloric acid solution with pepsin. Groups V and VI were submitted to 2 (GV) or 3 (GVI) weekly applications of 0.1N hydrochloric acid and treated with daily nitrate diluted in water. Group VII consisted of 10 animals submitted to 2 weekly applications of filtered water.

RESULTS:

No dysplasia, intra-epithelial neoplasia or invasive carcinomas were detected. Inflammatory changes were observed in varying degrees and mast cells were more common in Groups V and VI (p=0.006).

CONCLUSION:

The data of the current study could not corroborate the hypothesis that gastroesophageal and pharyngolaryngeal refluxes are carcinogenic factors to the laryngopharyngeal mucosa, and more studies are necessary in the future.
RESUMO

OBJETIVO:

Investigar a ação carcinogênica do ácido clorídrico, pepsina e nitrato de sódio na mucosa orofaríngea de ratos, simulando o refluxo do conteúdo gástrico à mucosa do faringo-laringea.

MÉTODOS:

Oitenta e dois ratos Wistar foram divididos em 7 grupos e submetidos a 2 ou 3 aplicações semanais de ácido clorídrico, pepsina e nitrato de sódio na mucosa orofaríngea durante 6 meses. Os grupos de estudo envolveram 12 animais cada. Os ratos nos grupos I e II foram submetidos à 2 (GI) ou 3 (GII) aplicações semanais de ácido clorídrico 0,1N. Nos grupos III e IV foram 2 (GIII) ou 3 (GIV) aplicações semanais de ácido clorídrico e pepsina. Nos grupos V e VI foram 2 (GV) ou 3 (GVI) aplicações semanais de ácido clorídrico além da oferta de nitrato diluído em água diariamente. Grupo VII era composto por 10 animais submetidos a 2 aplicações semanais de água filtrada.

RESULTADOS:

Não se observou displasia, neoplasia intra-epitelial ou neoplasia invasora. Alterações inflamatórias em graus variados foram observadas, com infiltrado mastocitário mais intenso nos grupos V e VI. (p=0,006).

CONCLUSÃO:

Os dados do presente estudo não confirmam a hipótese que o refluxo gastro-esofágico e faringo-laringeo são fatores carcinogênicos para a mucosa laringo-faringea e mais estudos são necessários no futuro.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Gastrointestinal Agents / Carcinogens / Carcinoma, Squamous Cell / Oropharyngeal Neoplasms / Hydrochloric Acid / Nitrates Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNICAMP/BR

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Full text: Available Index: LILACS (Americas) Main subject: Gastrointestinal Agents / Carcinogens / Carcinoma, Squamous Cell / Oropharyngeal Neoplasms / Hydrochloric Acid / Nitrates Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNICAMP/BR