Isolation and molecular identification of Leishmania chagasi from a bat (Carollia perspicillata) in northeastern Venezuela
Mem. Inst. Oswaldo Cruz
;
103(4): 412-414, June 2008. ilus
Article
in English
| LILACS
| ID: lil-486869
ABSTRACT
This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inoculated into the footpads of Balb/c mice, which subsequently developed a typical nodular leishmanial lesion; the parasites were confirmed as Leishmania by smear and histopathology. Molecular characterization of the parasites was performed by polymerase chain reaction with species-specific primers, kDNA restriction pattern following Hae III endonuclease digestion and dot blot hybridization using a kDNA probe. This report demonstrates that bats can be hosts for L. chagasi species and suggests the need for studies to determine whether they may be involved in foci of visceral leishmaniasis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Disease Reservoirs
/
Chiroptera
/
Leishmania infantum
/
DNA, Kinetoplast
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Venezuela
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
Affiliation country:
Venezuela
Institution/Affiliation country:
Geoclean C.A/VE
/
Universidad Central de Venezuela/VE
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