Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin / Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation
Arq. bras. med. vet. zootec
;
60(3): 766-768, jun. 2008. tab
Article
in Portuguese
| LILACS
| ID: lil-487927
ABSTRACT
The aim of this study was to compare three different selective enrichment broths Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of Salmonella Dublin (P<0,05), whereas RV broth incubated at 42ºC had a lower efficiency in the microbiological isolation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Salmonella
/
Salmonella Infections
/
Salmonella Infections, Animal
/
Cattle
/
Sodium Selenite
Limits:
Animals
Language:
Portuguese
Journal:
Arq. bras. med. vet. zootec
Journal subject:
Veterinary Medicine
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UNESP/BR
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