Plant regeneration of natural tetraploid Trifolium Hum pratense L
Biol. Res
;
41(1): 25-31, 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-490629
ABSTRACT
The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4 percent of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field.
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Index:
LILACS (Americas)
Main subject:
Plant Growth Regulators
/
Regeneration
/
Culture Media
/
Trifolium
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2008
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Ankara University/TR
/
Zonguldak Karaelmas University/TR
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