Effects of weight loss on liver and erythrocyte polyunsaturated fatty acid pattern and oxidative stress status in obese patients with non-alcoholic fatty liver disease
Biol. Res
;
41(1): 59-68, 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-490633
ABSTRACT
Our aim was to study the influence of weight loss on the fatty acid (FA) composition of liver and erythrocyte phospholipids and oxidative stress status in obese, non-alcoholic, fatty liver disease (NAFLD) patients. Seven obese NAFLD patients who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in roux and Y were studied immediately and 3 months after surgery. Seven non-obese patients who underwent anti-reflux surgery constituted the control group. Serum F2-isoprostane levels were measured by GS/NICI-MS/MS and FA composition was determined by GC. At the time of surgery, controls and obese patients exhibited a hepatic polyunsaturated fatty acid (PUFA) pattern that correlated with that of erythrocytes. Three months after surgery, NAFLD patients lost 21 percent of initial body weight; serum F2-isoprostane levels decreased by 76 percent; total PUFA, long-chain PUFA (LCPUFA), n-3 PUFA, and n-3 LCPUFA increased by 22, 29, 81, and 93 percent, respectively; n-6/n-3 LCPUFA ratio decreased by 51 percent; docosahexaenoic acid/docosapentaenoic acid ratio increased by 19-fold; and the n-3 product/precursor ratio (20 5 + 22 5 + 22 6)/18 3 increased by 164 percent (p<0.05). It is concluded that weight loss improves the n-3 LCPUFA status of obese patients in association with significant amelioration in the biomarkers of oxidative stress, membrane FA insaturation, and n-3 LCPUFA biosynthesis capacity, thus representing a central therapeutic issue in the improvement of obesity-related metabolic alterations involved in the mechanism of hepatic steatosis.
Full text:
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Index:
LILACS (Americas)
Main subject:
Oxidative Stress
/
Erythrocytes
/
Fatty Liver
/
Obesity
Type of study:
Observational study
/
Risk factors
Limits:
Adult
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Chile
/
Italy
Institution/Affiliation country:
Universidad de Chile Clinical Hospital/CL
/
Universidad de Chile/CL
/
Universitá degli Studi di Siena/IT
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