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Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: effect on triglyceride accumulation and cell proliferation
Julianelli, V. L; Guerra, L. N; Calvo, J. C.
  • Julianelli, V. L; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. AR
  • Guerra, L. N; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. AR
  • Calvo, J. C; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. AR
Biocell ; 31(2): 237-245, ago. 2007. graf
Article in English | LILACS | ID: lil-491530
ABSTRACT
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems to have an effect on this enzyme's activity and reduces it. The results presented herein contribute to the understanding of cell-cell communication in a model of a normal mammary gland.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Triglycerides / Culture Media, Conditioned / Epithelial Cells / Adipocytes, White / Mammary Glands, Animal Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Biocell Journal subject: C‚lulas Year: 2007 Type: Article Affiliation country: Argentina Institution/Affiliation country: Universidad de Buenos Aires/AR

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Full text: Available Index: LILACS (Americas) Main subject: Triglycerides / Culture Media, Conditioned / Epithelial Cells / Adipocytes, White / Mammary Glands, Animal Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Biocell Journal subject: C‚lulas Year: 2007 Type: Article Affiliation country: Argentina Institution/Affiliation country: Universidad de Buenos Aires/AR