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Genetic relationships between sympatric populations of Bacillus cereus and Bacillus thuringiensis, as revealed by rep-PCR genomic fingerprinting
Peruca, Ana Paula S; Vilas-Bôas, Gislayne T; Arantes, O. M. N.
  • Peruca, Ana Paula S; Universidade Estadual de Londrina. Departamento de Biologia Geral. Londrina. BR
  • Vilas-Bôas, Gislayne T; Universidade Estadual de Londrina. Departamento de Biologia Geral. Londrina. BR
  • Arantes, O. M. N; Universidade Estadual de Londrina. Departamento de Biologia Geral. Londrina. BR
Mem. Inst. Oswaldo Cruz ; 103(5): 497-500, Aug. 2008. tab, graf
Article in English | LILACS | ID: lil-491962
ABSTRACT
The bacterial strain Bacillus cereus is closely related to Bacillus thuringiensis, although any genetic relationship between the two strains is still in debate. Using rep-PCR genomic fingerprinting, we established the genetic relationships between Brazilian sympatric populations of B. cereus and B. thuringiensis simultaneously collected from two geographically separate sites. We observed the formation of both B. thuringiensis and B. cereus clusters, as well as strains of B. cereus that are more closely related to B. thuringiensis than to other B. cereus strains. In addition, lower genetic variability was observed among B. thuringiensis clusters compared to B. cereus clusters, indicating that either the two species should be categorized as separate or that B. thuringiensis may represent a clone from a B. cereus background.
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Full text: Available Index: LILACS (Americas) Main subject: Bacillus cereus / Bacillus thuringiensis / Genetic Variation / Polymerase Chain Reaction / DNA Fingerprinting Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Londrina/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacillus cereus / Bacillus thuringiensis / Genetic Variation / Polymerase Chain Reaction / DNA Fingerprinting Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Londrina/BR