Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples
Mem. Inst. Oswaldo Cruz
;
103(5): 463-467, Aug. 2008. tab
Article
in English
| LILACS
| ID: lil-491968
ABSTRACT
Comparison of the use of indirect immunofluorescence assay (IFA), immunochromatography assay (ICA-BD) and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus (HRSV) in 306 nasopharyngeal aspirates samples (NPA) was performed in order to assess their analytical performance. By comparing the results obtained using ICA-BD with those using IFA, we found relative indices of 85.0 percent for sensitivity and 91.2 percent for specificity, and the positive (PPV) and negative (NPV) predictive values were 85.0 percent and 91.2 percent, respectively. The relative indices for sensitivity and specificity as well as the PPV and NPV for RT-PCR were 98.0 percent, 89.0 percent, 84.0 percent and 99.0 percent, respectively, when compared to the results of IFA. In addition, comparison of the results of ICA-BD and those of RT-PCR yielded relative indices of 79.5 percent for sensitivity and 95.4 percent for specificity, as well as PPV and NPV of 92.9 percent and 86.0 percent, respectively. Although RT-PCR has shown the best performance, the substantial agreement between the ICA-BD and IFA results suggests that ICA-BD, also in addition to being a rapid and facile assay, could be suitable as an alternative diagnostic screening for HRSV infection in children.
Full text:
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Index:
LILACS (Americas)
Main subject:
Chromatography
/
Respiratory Syncytial Virus, Human
/
Respiratory Syncytial Virus Infections
/
Fluorescent Antibody Technique, Indirect
/
Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Child, preschool
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdade de Medicina de Jundiaí/BR
/
Instituto Adolfo Lutz/BR
/
Irmandade Santa Casa de Misericórdia de São Paulo/BR
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