Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages
Mem. Inst. Oswaldo Cruz
;
103(5): 501-503, Aug. 2008. tab
Article
in English
| LILACS
| ID: lil-491975
ABSTRACT
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
Full text:
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Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Polymerase Chain Reaction
/
DNA, Helminth
/
Microsatellite Repeats
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
Affiliation country:
France
Institution/Affiliation country:
Université de Perpignan/FR
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