Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia
Mem. Inst. Oswaldo Cruz
;
103(5): 493-496, Aug. 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-491980
ABSTRACT
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 percent). The Pvmsp-3 gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plasmodium vivax
/
Polymorphism, Genetic
/
DNA, Protozoan
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Colombia
Institution/Affiliation country:
Instituto Nacional de Salud/CO
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