Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones / Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
Rev. biol. trop
;
54(2): 253-256, jun. 2006.
Article
in Spanish
| LILACS
| ID: lil-492073
ABSTRACT
Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tetanus Toxin
/
Immunoenzyme Techniques
/
Clostridium tetani
/
Antibodies, Bacterial
/
Antigens, Bacterial
Type of study:
Diagnostic study
/
Etiology study
Limits:
Animals
Language:
Spanish
Journal:
Rev. biol. trop
Journal subject:
Biology
/
Tropical Medicine
Year:
2006
Type:
Article
Affiliation country:
Costa Rica
Institution/Affiliation country:
Universidad de Costa Rica/CR
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