Activity of antioxidant enzymes in seminal plasma and their relationship with lipid peroxidation of spermatozoa
Int. braz. j. urol
;
34(4): 485-491, July-Aug. 2008. tab
Article
in English
| LILACS
| ID: lil-493669
ABSTRACT
PURPOSE:
To determine the activity of seminal plasma catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) and their relationship with malondialdehyde (MDA), as a marker of lipid peroxidation, content of spermatozoa and seminal plasma in normozoospermic and asthenozoospermic males. MATERIALS ANDMETHODS:
Semen samples were obtained from 15 normozoospermic and 30 asthenozoospermic men.RESULTS:
We observed inverse correlations between activities of CAT (k/mL) and SOD (U/mL) in seminal plasma with MDA content of spermatozoa from normozoospermic samples (r =- 0.43, p < 0.05 and r =- 0.5, p < 0.05, respectively). Significant correlations were observed between total activity CAT (k/total seminal plasma) with total SOD (U/total seminal plasma) and GPX activity (mU/total seminal plasma) in seminal plasma from normozoospermic samples (r = 0.67, p = 0.008 and r = 0.455, p = 0.047, respectively). Furthermore, we found positive correlations between total activities of CAT, SOD and GPX with total content of MDA in seminal plasma (nmoL/total seminal plasma) from normozoospermic samples (r = 0.67, p = 0.003; r = 0.73, p = 0.003; r = 0.74, p = 0.004, respectively). In asthenozoospermic samples, there were no significant correlations observed between activities of CAT (k/mL), SOD (U/mL) and GPX (mU/mL) of seminal plasma with MDA content of spermatozoa. However, we found significant correlations between total activities of CAT (k/total seminal plasma) and SOD (U/total seminal plasma) with total content of MDA in seminal plasma (r = 0.4, p = 0.018 and r = 0.34, p = 0.03, respectively).CONCLUSION:
These findings indicate a protective role for antioxidant enzymes of seminal plasma against lipid peroxidation of spermatozoa in normozoospermic samples.
Full text:
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Index:
LILACS (Americas)
Main subject:
Semen
/
Spermatozoa
/
Lipid Peroxidation
/
Asthenozoospermia
Limits:
Adult
/
Humans
/
Male
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2008
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Hamadan University of Medical Sciences/IR
/
Kermanshah University of Medical Science/IR
/
Zahedan University of Medical Sciences/IR
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