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Dinámica de la descalcificación de tejido mineralizado de perros a través de microondas / Decalcification dynamic of dog mineralized tissue by microwaves
Pitol, Dimitrius Leonardo; Issa, Joáo Paulo Mardegan; Caetano, Flávio Henrique; Lunardi, Laurelúcia Orive.
  • Pitol, Dimitrius Leonardo; UNESP. Biosciences Institute. Molecular and Cellular Biology. BR
  • Issa, Joáo Paulo Mardegan; University of Sao Paulo. Faculty of Dentistry of Ribeirao Preto. BR
  • Caetano, Flávio Henrique; UNESP. Biosciences Institute. Molecular and Cellular Biology. BR
  • Lunardi, Laurelúcia Orive; University of Sao Paulo. Faculty of Dentistry of Ribeirao Preto. BR
Int. j. morphol ; 25(2): 309-313, jun. 2007. ilus, tab
Article in Spanish | LILACS | ID: lil-495939
ABSTRACT
The aim of this article is to present the decalcification process dynamic of mineralized tissue in dogs, teeth and jaw, comparing the traditional decalcification method, immersion, and microwave, immersion followed by irradiation using a domestic microwave oven, accompanying the liberation of calcium through spectrophotometer of atomic absorption. It was used as decalcified agent, EDTA solution or nitric acid. The results showed that with the use of nitric acid (5 percent), after 15 days, the irradiated fragments could be processed for histological analysis, otherwise the tooth not irradiated need to be submerged for 65 days. The EDTA decalcified action was slower than the nitric acid. The histological observations of the irradiated samples showed an excellent preservation of the morphological characteristics, independently of the decalcified agent used.
RESUMEN
El objetivo de este artículo fue presentar, un método de descalcificación dinámico de tejido mineralizado de perros, dientes y mandíbula, comparando el método de descalcificación tradicional e irradiación en horno de microondas, analizando la liberación de calcio en espectro fotómetro de absorción atómica. Usamos como agente descalcificante, solución de EDTA y acido nítrico. Los resultados mostraron que los fragmentos descalcificados con ácido nítrico después de 15 días, ya podían ser preparados para análisis histológico, el diente al ser irradiado tardó 65 días para ser descalcificado. El EDTA descalcificó lentamente, en relación al ácido nítrico. Las observaciones histológicas de las muestras irradiadas mostraron una excelente preservación de las características morfológicas independiente del agente descacificador usado.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Nitric Acid / Bone Demineralization Technique Limits: Animals Language: Spanish Journal: Int. j. morphol Journal subject: Anatomy Year: 2007 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: UNESP/BR / University of Sao Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Nitric Acid / Bone Demineralization Technique Limits: Animals Language: Spanish Journal: Int. j. morphol Journal subject: Anatomy Year: 2007 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: UNESP/BR / University of Sao Paulo/BR