Frozen Jaguar (Panthera onca) sperm capacitation and ability to penetrate zona free hamster oocytes / Capacitação espermática de sêmem congelado de onça pintada (Panthera onca) e ensaio de penetração em oócitos de hamster livres de zona pelúcida
Braz. j. vet. res. anim. sci
;
44(5): 337-344, 2007. tab
Article
in English
| LILACS
| ID: lil-497723
ABSTRACT
Assisted reproductive technologies in endangered species, such as artificial insemination, in vitro fertilization and embryo transfer, can be viewed as one potential approach for safeguarding species. Toward this aim, the objective of this study was to evaluate the fertility of frozen jaguar (Panthera onca) sperm and Tyrod's Talp PVA capacitation medium using the hamster zona free oocyte penetration assay. Ejaculates were collected from nine animals using electroejaculation and cryopreserved. Sperm capacitation was performed by swim-up technique using Tyrod's Talp PVA medium at room temperature. Penetration was considered when the spermatozoa head decondensation was visualized within the oocyte. This assay showed 15.4 % penetrations (350/2275 oocytes). Results of this study showed high sperm abnormalities, low sperm quality after cryopreservation, and low percentage of penetrations. However, the penetration results showed lhat the cryopreserved jaguar's semen can be used for artificial insemination, in vitro fertilization and intra cytoplasmatic sperm injection, supporting the semen bank creation for this specie.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oocytes
/
Semen Preservation
/
Sperm Capacitation
/
Insemination, Artificial
/
Fertilization in Vitro
/
Felis
Limits:
Animals
Language:
English
Journal:
Braz. j. vet. res. anim. sci
Journal subject:
Veterinary Medicine
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto de Tecnologia do Paraná/BR
/
Universidade Federal de Mato Grosso/BR
/
Universidade de São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS