Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis
Clinics
;
63(6): 815-820, 2008. ilus, graf
Article
in English
| LILACS
| ID: lil-497896
ABSTRACT
OBJECTIVE:
The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats.METHODS:
Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software.RESULTS:
Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.CONCLUSION:
The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.
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Index:
LILACS (Americas)
Main subject:
Bone Development
/
Plant Extracts
/
Cissus
/
Fetal Development
/
Alkanes
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2008
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Manipal College of Pharmaceutical Sciences/IN
/
Manipal University/IN
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