Influência da glutamina na regeneração hepática / Glutamine influence on liver regeneration

RESUMO

Objetivo: Este trabalho visa estabelecer a relação mais precisa do uso da glutamina na regeneração hepática. Métodos: Foram utilizados 37 ratos da linhagem Wistar, fêmeas, com idade de 120 dias e peso entre 345 ±80 gramas os quais foram divididos em um grupocontrole (C), com 17 animais, e um grupo de experimento (E), com 20 animais. Aproximadamente 70% do fígado foi cirurgicamente removido nos dois grupos. Administraram-se 50μg de glutamina/dia, via oral, diluído em 1 ml de água destilada, administrado por sondagem orogástrica diária no grupo de experimentação...

ABSTRACT

Objective: The aim of this work is to establish a precise relation between the use of glutamine and hepatic regeneration. Methods: 37 rats of the Wistar ancestry were used, females,with age of 120 days and weight between 345±80 grams, which were divided in a control group (C), with 17 animals, and an experimental group (E), with 20 animals. Approximately 70% of the liver was surgically removed in these two groups. 50 μg of utamine/day was managed, orally, diluted in 1 ml of distilled water, managed by daily gavage in the experimental group. In thecontrol group, distilled water was managed in equivalent volume. The contings were made in the periods of 24 hours and 7 days, by 3 methods: Formula of KWON et al to recognize volume gain, mitoses fi gures counting in 5 fi elds and PCNA positive nucleus percentage in 5 fields...