Effects of temperature, pH and additives on the activity of tannase produced by Paecilomyces variotii
Electron. j. biotechnol
;
10(2): 191-199, Apr. 15, 2007. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-499182
ABSTRACT
A biochemical characterization of the tannase from a Paecilomyces variotii strain isolated in Sao Paulo, Brazil was carried out. Paecilomyces variotii is a strain obtained from the screening of five hundred fungi that were tested for their production of tannase. The enzyme produced was partially purified using ammonium sulfate precipitation followed by ion exchange chromatography, diethylaminoethyl (DEAE)-Sepharose. Effects of temperature and pH on the activity of crude tannase crude and purified tannase was studied. Km was found to be 0.61 mol and Vmax = 0.55 U/mL. Temperature of 40 to 65øC and pH 4.5 to 6.5 were optimum for tannase activity and stability; it could find potential use in the food-processing industry. The effects of different inhibitors, surfactants and chelators on the enzyme activity were also studied
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paecilomyces
/
Carboxylic Ester Hydrolases
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Campinas Statue University/BR
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