Bovine kappa-casein gene polymorphism and its association with milk production traits
Genet. mol. biol
;
31(4): 893-897, Sept.-Dec. 2008. tab
Article
in English
| LILACS
| ID: lil-501445
ABSTRACT
Point mutations in exon IV of the bovine k-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3 percent agarose gels, and resolved by ethidium bromide staining. Allele A of the k-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and Tharparkar breeds were 0.758, 0.230 and 0.012, and 0.0.732, 0.250 and 0.018, respectively. The frequencies of alleles A and B in the Sahiwal and Tharparkar breeds were 0.873 and 0.127, and 0.857 and 0.143, respectively. Genotype BB of the kappa-casein gene had more influence on the monthly milk yield, 305-days milk yield, monthly solids-not-fat (SNF) yield, and monthly protein yield, in the Sahiwal cattle.
Full text:
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Index:
LILACS (Americas)
Main subject:
Polymorphism, Genetic
/
Cattle
/
Milk
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2008
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
National Dairy Research Institute/IN
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