Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad / In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) clones in myocardial cells from rodents of different susceptibility
Rev. biol. trop
;
55(1): 121-126, Mar. 2007. tab
Article
in Spanish
| LILACS
| ID: lil-501492
ABSTRACT
In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida Trypanosomatidae) clones in myocardial cells from rodents of different susceptibility. Two Trypanosoma cruzi isolates, TCR-4 from Costa Rica and UES-1 from El Salvador, were studied in vitro to compare their infectivity or resistance and intracellular replication in myocardial cells in three strains of mice and rats NGP white mice, C3 H mice and Sprague Dowley rats. Myocardial cells were cultured on coverslips at 37 degrees C in a humid 10% CO2 atmosphere and then infected at a ratio of one tripomastigote per cell. Samples were studied after 24, 72, 96 and 120 h of infection to determine parasite infection capacity and intracellular multiplication. Both parasites had the highest infection capacity in C3 H mice, followed by NGP mice cells with a very low infection rate. Lastly, almost no Trypanosoma cruzi multiplication was observed in Sprague Dowley rats, suggesting a strong natural resistance in this animal to both strains of the parasite. The UES-1 isolate presented higher multiplication and greater invasion than the TCR-4 strain, showing greater virulence of UES-1 in heart cells, at least in vitro.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Myocytes, Cardiac
Limits:
Animals
Country/Region as subject:
Central America
/
Costa Rica
/
El Salvador
Language:
Spanish
Journal:
Rev. biol. trop
Journal subject:
Biology
/
Tropical Medicine
Year:
2007
Type:
Article
Affiliation country:
Costa Rica
/
El salvador
Institution/Affiliation country:
Universidad de Costa Rica/CR
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