Evaluation of conventional serological test for the diagnosis of American cutaneous leishmaniasis
Bol. malariol. salud ambient
;
47(1): 55-62, 2007. tab
Article
in English
| LILACS
| ID: lil-503722
ABSTRACT
Three serological tests (ELISA, IFAT, DAT) were evaluated using sera from selected individuals with different American cutaneous leishmaniasis (ACL)clinical conditions. Reactivity in at least 2 of the 3 named tests was established as a criterion for declaring a patient as sero-positive. Prior to serological testing, people were diagnosed by clinical (presence of lesion = pl or scar=Sc), parasitological (presence of parasites =pp), immunological (LST) and molecular (PCR) methods. Fora statistical comparison of the evaluated tests 4 groups of people were made up1) patients with active leishmanial lesion (n=44; pl,pp,+LST,+PCR);2)patients who had recovered from leishmanial infection (n=43; Sc,+LST, +PCR); 3)asymptomatic individuals from endemic areas showing evidence of having contacted Leishmania -parasites (n=40;+LST,+PCR) and 4) Leishmania-negative people from the above localities where leishmaniasis is endemic and living under the same risk conditions, considered as healthy controls (n=104; -LST, -PCR). Considering the above-established criteria for sero-positives, the analysis of the results obtained with the 3 tests employed revealed very low sensitivity values. Seropositive figures of 50% were recorded for the first group, 9% for the secondgroup, and 12.5% for the third group. Statistical analysis also revealed a low positive predictive value (PPV= 0.73), a low negative predictive value (NPV= 0.49), and a low kappa coef ficient value (k= 0.114). The present results raise questions about the use of the 3 conventional serological tests compared here to detect ACL at any clinical condition.
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Index:
LILACS (Americas)
Main subject:
Parasitic Diseases
/
Serology
/
Leishmaniasis, Cutaneous
Type of study:
Diagnostic study
/
Prognostic study
Country/Region as subject:
South America
/
Venezuela
Language:
English
Journal:
Bol. malariol. salud ambient
Journal subject:
Social Sciences
/
Tropical Medicine
Year:
2007
Type:
Article
Affiliation country:
Venezuela
Institution/Affiliation country:
Universidad de Los Andes/VE
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