A model system to study antimicrobial strategies in endodontic biofilms
J. appl. oral sci
;
17(2): 87-91, Mar.-Apr. 2009. ilus
Article
in English
| LILACS
| ID: lil-503984
ABSTRACT
The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Enterococcus faecalis
/
Biofilms
/
Dental Pulp Cavity
/
Models, Biological
Limits:
Humans
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Goiás/BR
/
Federal University of Paraná/BR
/
Pontifical Catholic University of Rio Grande do Sul/BR
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