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Bioconversion of (+)- and (-)-alpha-pinene to (+)- and (-)-verbenone by plant cell cultures of Psychotria brachyceras and Rauvolfia sellowii
Limberger, Renata Pereira; Aleixo, Adriana Mendes; Fett-Neto, Arthur Germano; Henriques, Amélia T.
  • Limberger, Renata Pereira; Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós-Graduação em Ciências Farmacêuticas. Porto Alegre. BR
  • Aleixo, Adriana Mendes; Universidade Metodista de Piracicaba. Faculdade de Engenharia, Arquitetura e Urbanismo. Santa Barbara D'oeste. BR
  • Fett-Neto, Arthur Germano; Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre. BR
  • Henriques, Amélia T; Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós-Graduação em Ciências Farmacêuticas. Porto Alegre. BR
Electron. j. biotechnol ; 10(4): 500-507, oct. 2007. ilus, graf, tab
Article in English | LILACS | ID: lil-504125
ABSTRACT
This work describes the bioconversion of (-)- and (+)-alpha-pinene (2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene), targeted at the production of (-)- and (+)-verbenone (4,6,6-trimethyl-bicyclo (3.1.1) hept-3-en-2-one), respectively, using Psychotria brachyceras and Rauvolfia sellowii cell suspension cultures. P. brachyceras showed selectivity to (-)-alpha-pinene with 80.9 percent conversion (relative integrated area gas chromatography-mass spectrometry (GC-MS)) of (-)-verbenone in 10-day-incubation, whereas R. sellowii was able to convert both pinene enantiomers (37.6% conversion of (-)-verbenone in 7-day-incubation and 32.2% conversion of (+)-verbenone in 10-day-incubation). In both systems trans-verbenol was formed as main product and then slowly biocatalyzed to verbenone. Verbenone were also present among the autoxidation products during control experiments, but in much lower amounts and accompanied by several by-products, highlighting the usefulness of the biotransformation process.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Terpenes / Monoterpenes Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR / Universidade Metodista de Piracicaba/BR

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Full text: Available Index: LILACS (Americas) Main subject: Terpenes / Monoterpenes Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR / Universidade Metodista de Piracicaba/BR