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Arginine and glutamate levels in the gingival crevicular fluid from patients with chronic periodontitis
Téllez, Narda; Aguilera, Natalia; Quiñónez, Belkis; Silva, Elizabeth; González, Luis Enrique; Hernández, Luis.
  • Téllez, Narda; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
  • Aguilera, Natalia; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
  • Quiñónez, Belkis; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
  • Silva, Elizabeth; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
  • González, Luis Enrique; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
  • Hernández, Luis; Universidad de Los Andes. School of Medicine. Behavioral and Physiology Laboratory. Mérida. VE
Braz. dent. j ; 19(4): 318-322, 2008. ilus, graf, tab
Article in English | LILACS | ID: lil-504194
ABSTRACT
The objectives of this study were to determine arginine and glutamate levels in the gingival crevicular fluid (GCF) of adult chronic periodontitis patients versus periodontally healthy controls, and to compare two kinds of microdialysis probes normal and U-shaped probes. The analysis of GCF components was developed to improve the diagnosis of periodontal disease (PD). Proteolysis in the periodontal tissues increases the concentration of amino acids (aa) in the GCF and the levels of these aa may reveal PD features and stages. GCF samples were collected by microdialysis in situ from 5 periodontally affected sites (probing depth >5 mm, clinical attachment loss >3 mm) in 14 adult chronic periodontitis patients and from 14 adult periodontally healthy controls. Capillary zone electrophoresis coupled to laser induced fluorescence detection was used to measure concentration of arginine and glutamate in the GCF. Data were analyzed statistically by ANOVA and Tukey's post-hoc tests (?=0.05). Arginine concentration was increased (p<0.001) and glutamate concentration was decreased (p<0.001) in chronic periodontitis patients as compared to controls. There were no significant differences (p=0.069) between the normal and U-shaped probes. In conclusion, the increase of arginine and decrease of glutamate concentration in GCF were associated to the presence of periodontitis, and might be used as markers to recognize periodontally susceptible subjects as well as to evaluate the treatment course.
RESUMO
Os objetivos deste estudo foram determinar os níveis de arginina e glutamato no fluido gengival crevicular (FGC) em pacientes com periodontite crônica contra controles saudáveis e comparar dois tipos de cânulas de microdiálise normais e em forma de U. A análise dos componentes do FGC desenvolveu-se para melhorar o diagnóstico da doença periodontal (DP). A proteólise dos tecidos periodontais aumenta a concentração de aminoácidos (aa) no FGC e os níveis destes aa podem revelar as características e estágios da DP. Amostras de FGC foram obtidas pela técnica de microdiálise in situ de cinco zonas com o periodonto afetado (profundidade de sondagem >5 mm, perda da inserção clínica >3 mm) em 14 pacientes adultos com periodontite crônica e 14 controles saudáveis. Para medir a concentração de arginina e glutamato no GFC, usou-se a técnica de eletroforese capilar com detecção de fluorescência induzida por laser. Nos pacientes com periodontite crônica, a concentração de arginina aumentou significantemente (p<0.001), enquanto a de glutamato diminuiu significantemente (p<0.001) em comparação aos controles. Não houve diferenças significantes (p=0.069) entre as cânulas normais e as cânulas em forma de U. Conclui-se que o aumento da concentração de arginina e diminuição de glutamato no FGC estavam associados à presença de periodontite, e podem ser usados como marcadores para identificar pacientes suscetíveis à periodontite bem como avaliar a evolução do tratamento.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Arginine / Cysteine Endopeptidases / Gingival Crevicular Fluid / Glutamic Acid / Adhesins, Bacterial / Chronic Periodontitis Type of study: Observational study / Prognostic study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2008 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad de Los Andes/VE

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Full text: Available Index: LILACS (Americas) Main subject: Arginine / Cysteine Endopeptidases / Gingival Crevicular Fluid / Glutamic Acid / Adhesins, Bacterial / Chronic Periodontitis Type of study: Observational study / Prognostic study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2008 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad de Los Andes/VE