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Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01 / Identificação de um metabólito antifúngico produzido pela cepa Actinomyces A01
Lu, Cai Ge; Liu, Wei Cheng; Qiu, Ji Yan; Wang, Hui Min; Ting, Liu; Liu, De Wen.
  • Lu, Cai Ge; China Agricultural University. Department of Plant Pathology. Beijing. CN
  • Liu, Wei Cheng; Beijing Academy of Agriculture and Forestry Sciences. Institute of Plant and Environment Protection. CN
  • Qiu, Ji Yan; Beijing Academy of Agriculture and Forestry Sciences. Institute of Plant and Environment Protection. CN
  • Wang, Hui Min; China Agricultural University. Department of Plant Pathology. Beijing. CN
  • Ting, Liu; Beijing Academy of Agriculture and Forestry Sciences. Institute of Plant and Environment Protection. CN
  • Liu, De Wen; Beijing Academy of Agriculture and Forestry Sciences. Institute of Plant and Environment Protection. CN
Braz. j. microbiol ; 39(4): 701-707, Dec. 2008. ilus, tab
Article in English | LILACS | ID: lil-504310
ABSTRACT
Actinomyces strain A01 was isolated from soil of a vegetable field in the suburb of Beijing, China. According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain A01 was identified as Streptomyces lydicus. In the antimicrobial spectrum test strain A01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as Fusarium oxysporum, Botrytis cinerea, Monilinia laxa, etc. However, no antibacterial activity was found. In pot experiments in greenhouse, the development of tomato gray mold was markedly suppressed by treatment with the fermentation broth of the strain A01, and the control efficacy was higher than those of Pyrimethanil and Polyoxin. A main antifungal compound (purity 99.503 percent) was obtained from the fermentation broth of strain A01 using column chromatography and HPLC. The chemical structural analysis with UV, IR, MS, and NMR confirmed that the compound produced by the strain A01 is natamycin, a polyene antibiotic produced by S. chattanovgensis, S. natalensis, and S. gilvosporeus, widely used as a natural biological preservative for food according to previous reports. The present study revealed a new producing strain of natamycin and its potential application as a biological control agent for fungal plant diseases.
RESUMO
A cepa Actinomyces A01 foi isolada do solo de um campo agrícola no subúrbio de Beijing, China. De acordo com as características morfológicas, culturais, fisiológicas e bioquímicas, e análise da sequência 16S rDNA , a cepa A01 foi identificada como Streptomyces lydicus. Nos testes de espectro antimicrobiano, a cepa A01 apresentou atividade inibitória intensa e estável contra vários fungos patogênicos para plantas, como Fusarium oxysporum, Botrytis cinerea, Monilia laxa, etc. Entretanto, não foi encontrada atividade antibacteriana. Em experimentos em estufas, o desenvolvimento do fungo cinza do tomate foi fortemente inibido pelo tratamento com o caldo de fermentação da cepa A01, com eficiência superior à do pyremethanil e polyoxin. Por cromatografia em coluna e HPLC, obteve-se um composto fúngico (pureza 99,503 por cento), cuja análise estrutural por UV, IR, MS e NMR revelou ser natamicina, um antibiótico polienico produzido por S. chattanovgensis, S. natalensis e S.gilvosporeus, empregado como conservador biológico natural em alimentos. O presente estudo relata a detecção de uma nova cepa produtora de natamicina e sua aplicação potencial como um agente de controle biológico de doenças fúngicas em plantas.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Plant Diseases / Actinomyces / Base Sequence / Pest Control, Biological / Mitosporic Fungi / Fermentation / Mycoses / Antifungal Agents Type of study: Diagnostic study / Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2008 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Beijing Academy of Agriculture and Forestry Sciences/CN / China Agricultural University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Plant Diseases / Actinomyces / Base Sequence / Pest Control, Biological / Mitosporic Fungi / Fermentation / Mycoses / Antifungal Agents Type of study: Diagnostic study / Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2008 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Beijing Academy of Agriculture and Forestry Sciences/CN / China Agricultural University/CN