Estimating genomic instability mediated by Alu retroelements in breast cancer
Genet. mol. biol
;
32(1): 25-31, 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-505769
ABSTRACT
Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Breast Neoplasms
/
Carcinoma, Ductal, Breast
/
Alu Elements
/
Genomic Instability
Type of study:
Qualitative research
Limits:
Female
/
Humans
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdade de Medicina/BR
/
Universidade Estadual Paulista Júlio De Mesquita Filho/BR
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