Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Genet. mol. biol
;
32(1): 155-158, 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-505775
ABSTRACT
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
Full text:
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Index:
LILACS (Americas)
Main subject:
DNA Damage
/
Comet Assay
/
Erythrocytes
/
Fishes
Limits:
Animals
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista Júlio de Mesquita Filho/BR
/
Universidade Federal do Pará/BR
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