Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
J. venom. anim. toxins incl. trop. dis
;
15(1): 28-42, 2009. ilus, graf
Article
in English
| LILACS
| ID: lil-508228
ABSTRACT
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively secarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 miug/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50 2 miug/mL) than B. aftematus (CC50 5.8 miug/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mediated this cell line destruction. The current study aimed to provide new information on the citotoxicity meohanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Crotalid Venoms
Limits:
Animals
Country/Region as subject:
South America
/
Argentina
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Argentina
Institution/Affiliation country:
Northeast National University/AR
/
University of Buenos Aires/AR
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