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Lactobacillus delbrueckii UFV-H2b20 induces type 1 cytokine production by mouse cells in vitro and in vivo
Neumann, E; Ramos, M. G; Santos, L. M; Rodrigues, A. C. P; Vieira, E. C; Afonso, L. C. C; Nicoli, J. R; Vieira, L. Q.
  • Neumann, E; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
  • Ramos, M. G; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
  • Santos, L. M; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
  • Rodrigues, A. C. P; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
  • Vieira, E. C; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
  • Afonso, L. C. C; Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto. BR
  • Nicoli, J. R; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte. BR
  • Vieira, L. Q; Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte. BR
Braz. j. med. biol. res ; 42(4): 358-367, Apr. 2009. graf
Article in English | LILACS | ID: lil-509175
ABSTRACT
Lactobacillus delbrueckii UFV-H2b20 has been shown to increase clearance of bacteria injected into the blood of germ-free mice. Moreover, it induces the production of type 1 cytokines by human peripheral mononuclear cells. The objective of the present study was to investigate the production of inflammatory cytokines [interleukin-12 (IL-12 p40), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)] triggered in vitro by live, heat-killed or lysozyme-treated L. delbrueckii UFV-H2b20 and in vivo by a live preparation. Germ-free, L. delbrueckii-monoassociated and lipopolysaccharide (LPS)-resistant C3H/HeJ mice were used as experimental models. UFV-H2b20 induced the production of IL-12 p40 and TNF-α by peritoneal cells and IFN-γ by spleen cells from germ-free or monoassociated Swiss/NIH mice and LPS-hyporesponsive mice (around 40 ng/mL for IL-12 p40, 200 pg/mL for TNF-α and 10 ng/mL for IFN-γ). Heat treatment of L. delbrueckii did not affect the production of these cytokines. Lysozyme treatment decreased IL-12 p40 production by peritoneal cells from C3H/HeJ mice, but did not affect TNF-α production by these cells or IFN-γ production by spleen cells from the same mouse strain. TNF-α production by peritoneal cells from Swiss/NIH L. delbrueckii-monoassociated mice was inhibited by lysozyme treatment. When testing IL-12 p40 and IFN-γ levels in sera from germ-free or monoassociated Swiss/NIH mice systemically challenged with Escherichia coli we observed that IL-12 p40 was produced at marginally higher levels by monoassociated mice than by germ-free mice (40 vs 60 ng/mL), but IFN-γ was produced earlier and at higher levels by monoassociated mice (monoassociated 4 and 14 ng/mL 4 and 8 h after infection, germfree 0 and 7.5 ng/mL at the same times). These results show that L. delbrueckii UFV-H2b20 stimulates the production of type 1 cytokines in vitro and in vivo, therefore suggesting...
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Full text: Available Index: LILACS (Americas) Main subject: Interferon-gamma / Tumor Necrosis Factor-alpha / Macrophages, Peritoneal / Lactobacillus delbrueckii Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2009 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR / Universidade Federal de Ouro Preto/BR

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Full text: Available Index: LILACS (Americas) Main subject: Interferon-gamma / Tumor Necrosis Factor-alpha / Macrophages, Peritoneal / Lactobacillus delbrueckii Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2009 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR / Universidade Federal de Ouro Preto/BR