Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
Braz. arch. biol. technol
;
52(2): 265-270, Mar.-Apr. 2009. ilus, graf
Article
in English
| LILACS
| ID: lil-513245
ABSTRACT
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2009
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Northwest A&F University Yangling/CN
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