Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Genet. mol. biol
;
32(2): 337-339, 2009. tab
Article
in English
| LILACS
| ID: lil-513962
ABSTRACT
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
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Index:
LILACS (Americas)
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Brazil
/
Italy
Institution/Affiliation country:
Seção de Orquidário do Estado/BR
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade de São Paulo/BR
/
Università degli Studi di Napoli Federico II/IT
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