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Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum
Monteiro, Rita; Carvalho, Cláudia; Collares-Pereira, Maria João.
  • Monteiro, Rita; Universidade de Lisboa. Faculdade de Ciências. Departamento de Biologia Animal. Centro de Biologia Ambiental. Lisboa. PT
  • Carvalho, Cláudia; Universidade de Lisboa. Faculdade de Ciências. Departamento de Biologia Animal. Centro de Biologia Ambiental. Lisboa. PT
  • Collares-Pereira, Maria João; Universidade de Lisboa. Faculdade de Ciências. Departamento de Biologia Animal. Centro de Biologia Ambiental. Lisboa. PT
Genet. mol. biol ; 32(2): 268-275, 2009. ilus, mapas, tab
Article in English | LILACS | ID: lil-513968
ABSTRACT
This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M15SM3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both between and within species the genome of I. almacai was smaller than that of I. lusitanicum (mean values 2.61 and 2.93 pg, respectively), which presented a clear interpopulational variability (mean values ranging from 2.72 to 3.00 pg). These data allowed the distinction of both taxa and confirmed the existence of two well differentiated groups within I. lusitanicum one that includes the populations from the right bank of the Tejo and Samarra drainages, and another that reunites the southern populations. The peculiar differences between the two species, presently listed as "Critically Endangered", reinforced the importance of this study for future conservation plans.

Full text: Available Index: LILACS (Americas) Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2009 Type: Article / Project document Affiliation country: Portugal Institution/Affiliation country: Universidade de Lisboa/PT

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Full text: Available Index: LILACS (Americas) Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2009 Type: Article / Project document Affiliation country: Portugal Institution/Affiliation country: Universidade de Lisboa/PT