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Avaliação laboratorial da doença residual mínima na leucemia mielóide crônica por Real-Time PCR: [revisão] / Evaluation diagnosis of minimal residual disease in chronic myeloid leukemia by Real-Time PCR: [review]
Grando, Allyne Cristina; Wagner, Sandrine Comparsi.
  • Grando, Allyne Cristina; s.af
  • Wagner, Sandrine Comparsi; FEEVALE. Novo Hamburgo. BR
J. bras. patol. med. lab ; 44(6): 433-440, dez. 2008.
Article in Portuguese | LILACS | ID: lil-515120
RESUMO
A leucemia mielóide crônica (LMC) representa 15 por cento das leucemias e apresenta três fases crônica, acelerada e crise blástica. A partir da análise citogenética, pode ser identificado o cromossomo Philadelphia, característico da LMC. O transplante de células-tronco é o único tratamento curativo, mas é acompanhado de altas taxas de morbimortalidade, dificultando sua aplicação. A doença residual mínima é de grande importância para avaliar a resposta ao tratamento, tanto na verificação de doença residual, quanto na identificação de pacientes com alto risco de recaída. Muitas técnicas específicas têm sido introduzidas para detectar as translocações ou os produtos do cromossomo Philadelphia. A mais sensível é a Real-Time PCR, que detecta uma célula leucêmica em 10(5) células normais. O objetivo deste trabalho foi realizar uma revisão bibliográfica sobre a LMC, dando ênfase à utilização da técnica por Real-Time PCR.
ABSTRACT
Chronic myeloid leukemia (CML) represents about 15 percent of all leukemias and has three phases the chronic phase, accelerated phase and blast crisis. After cytogenetic analysis, the Philadelphia chromosome, characteristic of CML, can be identificated. Stem cell transplantation is the only curative treatment for CML, but it is accompanied by high levels of morbimortality, difficulting its application. The minimal residual disease is very important for the evaluation of the response to treatment, to verify the residual disease and also to identify patients with a high risk of relapse. Many specific techniques have been introduced for the detection of translocations or products of the Philadelphia chromosome; the most sensitive being Real-Time PCR which detects 1 leukemia cell in 10(5) normal cells. The aim of this study was to perform a bibliographic review of CML, with emphasis on the utilization of the Real-Time PCR technique.
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Full text: Available Index: LILACS (Americas) Main subject: Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Neoplasm, Residual / Reverse Transcriptase Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Limits: Humans Language: Portuguese Journal: J. bras. patol. med. lab Journal subject: Pathology Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: FEEVALE/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Neoplasm, Residual / Reverse Transcriptase Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Limits: Humans Language: Portuguese Journal: J. bras. patol. med. lab Journal subject: Pathology Year: 2008 Type: Article Affiliation country: Brazil Institution/Affiliation country: FEEVALE/BR