Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins
Mem. Inst. Oswaldo Cruz
;
104(3): 427-433, May 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-517006
ABSTRACT
Fifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I A). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I A of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aspergillus fumigatus
/
Genetic Variation
/
Environment
Country/Region as subject:
South America
/
Argentina
/
Europa
/
Mexico
/
Peru
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Argentina
/
France
/
Mexico
Institution/Affiliation country:
Hospital General Dr. Manuel Gea González/MX
/
INEI ANLIS Dr. Carlos G. Malbrán/AR
/
Instituto Politécnico Nacional/MX
/
Lille 2 University/FR
/
Universidad Nacional Autónoma de México/MX
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