HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Mem. Inst. Oswaldo Cruz
;
104(3): 399-410, May 2009. ilus
Article
in English
| LILACS
| ID: lil-517020
ABSTRACT
Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Herpesvirus 1, Human
/
Gene Transfer Techniques
/
Transgenes
/
Genetic Vectors
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
France
Institution/Affiliation country:
Université de Lyon/FR
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