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HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Epstein, Alberto Luis.
  • Epstein, Alberto Luis; Université de Lyon. Université Claude Bernard Lyon 1. Centre de Génétique Moléculaire et Cellulaire. Villeurbanne. FR
Mem. Inst. Oswaldo Cruz ; 104(3): 399-410, May 2009. ilus
Article in English | LILACS | ID: lil-517020
ABSTRACT
Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
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Full text: Available Index: LILACS (Americas) Main subject: Herpesvirus 1, Human / Gene Transfer Techniques / Transgenes / Genetic Vectors Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2009 Type: Article / Project document Affiliation country: France Institution/Affiliation country: Université de Lyon/FR

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Full text: Available Index: LILACS (Americas) Main subject: Herpesvirus 1, Human / Gene Transfer Techniques / Transgenes / Genetic Vectors Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2009 Type: Article / Project document Affiliation country: France Institution/Affiliation country: Université de Lyon/FR