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Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary TB / Associação entre coleta de escarro guiada e taxas de contaminação de culturas para o diagnóstico de TB pulmonar
Maciel, Ethel Leonor Noia; Prado, Thiago Nascimento do; Peres, Renata Lyrio; Palaci, Moises; Johnson, John L; Dietze, Reynaldo.
  • Maciel, Ethel Leonor Noia; Universidade Federal do Espírito Santo. Discipline of Epidemiology. Vitória. BR
  • Prado, Thiago Nascimento do; Universidade Federal do Espírito Santo. Postgraduate Program in Infectious Diseases. Vitória. BR
  • Peres, Renata Lyrio; Universidade Federal do Espírito Santo. Postgraduate Program in Infectious Diseases. Vitória. BR
  • Palaci, Moises; Universidade Federal do Espírito Santo. Infectious Disease Center. Laboratory of Microbiology. Vitória. BR
  • Johnson, John L; Case Western Reserve University. Tuberculosis Research Unit. Clinical Trial Center. Cleveland. US
  • Dietze, Reynaldo; Universidade Federal do Espírito Santo. Infectious Disease Center. Vitória. BR
J. bras. pneumol ; 35(5): 460-463, maio 2009. tab
Article in English, Portuguese | LILACS | ID: lil-517072
ABSTRACT
A comparative study to evaluate contamination in cultures of morning sputum samples, comparing those collected at home under currently recommended conditions and those collected under supervision after patient orientation and education. The home and supervised collection groups produced 43 and 76 sputum samples, respectively.The contamination rate was nearly 3-times higher among samples collected at home than among those collected under supervision (37% vs. 13%, p < 0.05; OR = 0.25). The simple educational and hygiene measures described can decrease the contamination rate among sputum samples collected for diagnostic culture.
RESUMO
Realizou-se um estudo comparativo para avaliar a contaminação em culturas de amostras matinais de escarro coletadas em domicílio sob condições recomendadas atualmente e amostras coletadas sob supervisão após orientação e educação dos pacientes. Os grupos de coleta domiciliar e supervisionada produziram 43 e 76 amostras, respectivamente. A taxa de contaminação foi aproximadamente 3 vezes maior nas amostras coletadas em domicílio do que naquelas coletadas sob supervisão (37% vs.13%; p < 0,05; OR = 0,25). As simples medidas educacionais e de higiene descritas podem reduzir a taxa de contaminação de amostras de escarro coletadas para culturas diagnósticas.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Specimen Handling / Sputum / Tuberculosis, Pulmonary Type of study: Diagnostic study Limits: Adult / Female / Humans / Male Language: English / Portuguese Journal: J. bras. pneumol Journal subject: Pulmonary Disease (Specialty) Year: 2009 Type: Article / Project document Affiliation country: Brazil / United States Institution/Affiliation country: Case Western Reserve University/US / Universidade Federal do Espírito Santo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Specimen Handling / Sputum / Tuberculosis, Pulmonary Type of study: Diagnostic study Limits: Adult / Female / Humans / Male Language: English / Portuguese Journal: J. bras. pneumol Journal subject: Pulmonary Disease (Specialty) Year: 2009 Type: Article / Project document Affiliation country: Brazil / United States Institution/Affiliation country: Case Western Reserve University/US / Universidade Federal do Espírito Santo/BR